Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides
Identifieur interne : 001D16 ( Main/Exploration ); précédent : 001D15; suivant : 001D17Epitope mapping of cross-reactive monoclonal antibodies specific for the influenza A virus PA and PB2 polypeptides
Auteurs : M. Ochoa [Espagne] ; J. Bárcena [Espagne] ; S. De La Luna [Espagne] ; J. A. Melero [Espagne] ; A. R. Douglas [Royaume-Uni] ; A. Nieto [Espagne] ; J. Ortín [Espagne] ; J. J. Skehel [Royaume-Uni] ; A. Portela [Espagne]Source :
- Virus Research [ 0168-1702 ] ; 1995.
English descriptors
- Teeft :
- Amino, Amino acids, Antibody, Antigenic, Antigenic areas, Assay, Competitive elisas, Elisa, Epitope, Influenza, Influenza virus, Influenza viruses, Mabs, Molecular weights, Monoclonal, Monoclonal antibodies, Ochoa, Plasmid, Polymerase, Polypeptide, Protein fragments, Protein regions, Syncytial virus, Viral, Viral strains, Virol, Virology, Virus, Virus research.
Abstract
Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.
Url:
DOI: 10.1016/0168-1702(95)00039-S
Affiliations:
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De La Luna</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Melero, J A" sort="Melero, J A" uniqKey="Melero J" first="J. A." last="Melero">J. A. Melero</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Douglas, A R" sort="Douglas, A R" uniqKey="Douglas A" first="A. R." last="Douglas">A. R. Douglas</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA</wicri:regionArea><wicri:noRegion>London NW7 1AA</wicri:noRegion></affiliation></author><author><name sortKey="Nieto, A" sort="Nieto, A" uniqKey="Nieto A" first="A." last="Nieto">A. Nieto</name><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología (CSIC), Universidad Autónoma, Cantoblanco 28049, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Ortin, J" sort="Ortin, J" uniqKey="Ortin J" first="J." last="Ortín">J. 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Portela</name><affiliation></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Salud Carlos III, Centro Nacional de Biología Celular y Retrovirus, Majadahonda 28220, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Research</title><title level="j" type="abbrev">VIRUS</title><idno type="ISSN">0168-1702</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="305">305</biblScope><biblScope unit="page" to="315">315</biblScope></imprint><idno type="ISSN">0168-1702</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-1702</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acids</term><term>Antibody</term><term>Antigenic</term><term>Antigenic areas</term><term>Assay</term><term>Competitive elisas</term><term>Elisa</term><term>Epitope</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Mabs</term><term>Molecular weights</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Ochoa</term><term>Plasmid</term><term>Polymerase</term><term>Polypeptide</term><term>Protein fragments</term><term>Protein regions</term><term>Syncytial virus</term><term>Viral</term><term>Viral strains</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Virus research</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Characterization of the epitopes recognized by 21 monoclonal antibodies (MAbs) specific for the influenza A virus PA (13 MAbs) and PB2 (8 MAbs) polypeptides (Bárcena et al. (1994) J. Virol. 68, 6900–6909) raised against denatured polypeptides produced in E. coli is described. MAbs were characterized by: (1) competitive binding ELISAs; (2) mapping of the protein regions that specify their binding sites; and (3) analyses of their ability to recognize the corresponding viral protein in a number of viral isolates. Five and three nondashoverlapping antigenic areas were defined by the anti-PA and anti-PB2 MAbs, respectively. Five of the anti-PA MAbs recognized antigenic determinants located within the amino-terminal 157 amino acids of the PA protein, and 6 others reacted strongly with a PA fragment comprising the first 236 amino acids. All 8 anti-PB2 antibodies reacted strongly with a polypeptide fragment containing amino acids 1–113 of the PB2 protein. Analyses of the reactivities of 4 anti-P antibodies with 23 influenza A virus reference strains isolated over a period of 61 years and recovered from humans, pigs, birds and horses, showed that the epitopes were conserved among all viral isolates. The application of these antibodies as research and diagnostic tools is discussed.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>Royaume-Uni</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Ochoa, M" sort="Ochoa, M" uniqKey="Ochoa M" first="M." last="Ochoa">M. Ochoa</name></region><name sortKey="Barcena, J" sort="Barcena, J" uniqKey="Barcena J" first="J." last="Bárcena">J. Bárcena</name><name sortKey="De La Luna, S" sort="De La Luna, S" uniqKey="De La Luna S" first="S." last="De La Luna">S. De La Luna</name><name sortKey="Melero, J A" sort="Melero, J A" uniqKey="Melero J" first="J. A." last="Melero">J. A. Melero</name><name sortKey="Nieto, A" sort="Nieto, A" uniqKey="Nieto A" first="A." last="Nieto">A. Nieto</name><name sortKey="Ortin, J" sort="Ortin, J" uniqKey="Ortin J" first="J." last="Ortín">J. Ortín</name><name sortKey="Portela, A" sort="Portela, A" uniqKey="Portela A" first="A." last="Portela">A. Portela</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Douglas, A R" sort="Douglas, A R" uniqKey="Douglas A" first="A. R." last="Douglas">A. R. Douglas</name></noRegion><name sortKey="Skehel, J J" sort="Skehel, J J" uniqKey="Skehel J" first="J. J." last="Skehel">J. J. Skehel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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